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flow cytometry analysis permeabilizing solution  (Becton Dickinson)

 
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    Becton Dickinson flow cytometry analysis permeabilizing solution
    Effect of estradiol on cell cycle. Flow <t>cytometry</t> analysis of propidium iodide-labeled EPN (A) or CPEC (B) cells was done, as described in Methods. Quiescent cells were left under basal conditions or treated with estradiol (20 nM; E 2 ) for the indicated times. Histograms represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after 24 or 48 h of hormonal treatment. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05 for each experimental point vs. the corresponding untreated control.
    Flow Cytometry Analysis Permeabilizing Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+analysis+permeabilizing+solution/flow+cytometry+permeabilizing+solution/pmc06384260-76-12-24
    Average 90 stars, based on 1 article reviews
    flow cytometry analysis permeabilizing solution - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells"

    Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2019.00028

    Effect of estradiol on cell cycle. Flow cytometry analysis of propidium iodide-labeled EPN (A) or CPEC (B) cells was done, as described in Methods. Quiescent cells were left under basal conditions or treated with estradiol (20 nM; E 2 ) for the indicated times. Histograms represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after 24 or 48 h of hormonal treatment. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05 for each experimental point vs. the corresponding untreated control.
    Figure Legend Snippet: Effect of estradiol on cell cycle. Flow cytometry analysis of propidium iodide-labeled EPN (A) or CPEC (B) cells was done, as described in Methods. Quiescent cells were left under basal conditions or treated with estradiol (20 nM; E 2 ) for the indicated times. Histograms represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after 24 or 48 h of hormonal treatment. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05 for each experimental point vs. the corresponding untreated control.

    Techniques Used: Flow Cytometry, Labeling, Control

    Effect of both estradiol and pasireotide on cell cycle. Flow cytometry analysis of propidium iodide-labelled EPN (A) or CPEC (B) was done, as described in Methods. In panel (A,B) , quiescent cells were left under untreated or treated for 24 h with 0,1 μM pasireotide, in the absence or presence of 20 nM estradiol. Histograms in panel (A,B) represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after the indicated treatments. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05; ∗∗ p < 0,01. In panel (C) , quiescent EPN (left section) or CPEC (right section) cells were left untreated or treated for 24 h with 0,1 μM pasireotide, in the absence or presence of 20 nM estradiol. Lysate proteins were separated by SDS-PAGE, transferred to PDVF membrane and filters were then analyzed by Western blot, using the anti-activated caspase 3 or anti-tubulin antibodies.
    Figure Legend Snippet: Effect of both estradiol and pasireotide on cell cycle. Flow cytometry analysis of propidium iodide-labelled EPN (A) or CPEC (B) was done, as described in Methods. In panel (A,B) , quiescent cells were left under untreated or treated for 24 h with 0,1 μM pasireotide, in the absence or presence of 20 nM estradiol. Histograms in panel (A,B) represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after the indicated treatments. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05; ∗∗ p < 0,01. In panel (C) , quiescent EPN (left section) or CPEC (right section) cells were left untreated or treated for 24 h with 0,1 μM pasireotide, in the absence or presence of 20 nM estradiol. Lysate proteins were separated by SDS-PAGE, transferred to PDVF membrane and filters were then analyzed by Western blot, using the anti-activated caspase 3 or anti-tubulin antibodies.

    Techniques Used: Flow Cytometry, Labeling, SDS Page, Membrane, Western Blot

    Related Articles

    Flow Cytometry:

    Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells
    Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).

    FACS:

    Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells
    Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).

    Labeling:

    Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells
    Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).

    Control:

    Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells
    Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).

    SDS Page:

    Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells
    Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).

    Membrane:

    Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells
    Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).

    Western Blot:

    Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells
    Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).



    Similar Products

    flow cytometry analysis permeabilizing solution  (Becton Dickinson)
    90
    Becton Dickinson flow cytometry analysis permeabilizing solution
    Effect of estradiol on cell cycle. Flow <t>cytometry</t> analysis of propidium iodide-labeled EPN (A) or CPEC (B) cells was done, as described in Methods. Quiescent cells were left under basal conditions or treated with estradiol (20 nM; E 2 ) for the indicated times. Histograms represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after 24 or 48 h of hormonal treatment. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05 for each experimental point vs. the corresponding untreated control.
    Flow Cytometry Analysis Permeabilizing Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flow+cytometry+analysis+permeabilizing+solution/flow+cytometry+permeabilizing+solution/pmc06384260-76-12-24
    Average 90 stars, based on 1 article reviews
    flow cytometry analysis permeabilizing solution - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of estradiol on cell cycle. Flow cytometry analysis of propidium iodide-labeled EPN (A) or CPEC (B) cells was done, as described in Methods. Quiescent cells were left under basal conditions or treated with estradiol (20 nM; E 2 ) for the indicated times. Histograms represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after 24 or 48 h of hormonal treatment. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05 for each experimental point vs. the corresponding untreated control.

    Journal: Frontiers in Pharmacology

    Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells

    doi: 10.3389/fphar.2019.00028

    Figure Lengend Snippet: Effect of estradiol on cell cycle. Flow cytometry analysis of propidium iodide-labeled EPN (A) or CPEC (B) cells was done, as described in Methods. Quiescent cells were left under basal conditions or treated with estradiol (20 nM; E 2 ) for the indicated times. Histograms represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after 24 or 48 h of hormonal treatment. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05 for each experimental point vs. the corresponding untreated control.

    Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).

    Techniques: Flow Cytometry, Labeling, Control

    Effect of both estradiol and pasireotide on cell cycle. Flow cytometry analysis of propidium iodide-labelled EPN (A) or CPEC (B) was done, as described in Methods. In panel (A,B) , quiescent cells were left under untreated or treated for 24 h with 0,1 μM pasireotide, in the absence or presence of 20 nM estradiol. Histograms in panel (A,B) represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after the indicated treatments. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05; ∗∗ p < 0,01. In panel (C) , quiescent EPN (left section) or CPEC (right section) cells were left untreated or treated for 24 h with 0,1 μM pasireotide, in the absence or presence of 20 nM estradiol. Lysate proteins were separated by SDS-PAGE, transferred to PDVF membrane and filters were then analyzed by Western blot, using the anti-activated caspase 3 or anti-tubulin antibodies.

    Journal: Frontiers in Pharmacology

    Article Title: Estrogens Modulate Somatostatin Receptors Expression and Synergize With the Somatostatin Analog Pasireotide in Prostate Cells

    doi: 10.3389/fphar.2019.00028

    Figure Lengend Snippet: Effect of both estradiol and pasireotide on cell cycle. Flow cytometry analysis of propidium iodide-labelled EPN (A) or CPEC (B) was done, as described in Methods. In panel (A,B) , quiescent cells were left under untreated or treated for 24 h with 0,1 μM pasireotide, in the absence or presence of 20 nM estradiol. Histograms in panel (A,B) represent the % counts/FL2 areas of labeled cells in the different cell cycle phases after the indicated treatments. Means and SEMs are shown. n represents the number of experiments. ∗ p < 0,05; ∗∗ p < 0,01. In panel (C) , quiescent EPN (left section) or CPEC (right section) cells were left untreated or treated for 24 h with 0,1 μM pasireotide, in the absence or presence of 20 nM estradiol. Lysate proteins were separated by SDS-PAGE, transferred to PDVF membrane and filters were then analyzed by Western blot, using the anti-activated caspase 3 or anti-tubulin antibodies.

    Article Snippet: Permeabilization was performed by incubating 10 6 cells in 0,5 ml of flow cytometry analysis (FACS) permeabilizing solution, which contains RNAses and propidium iodide (Beckton Dickinson).

    Techniques: Flow Cytometry, Labeling, SDS Page, Membrane, Western Blot